The development of this bioprocess occurred in Experimental Base of Embrapa MeioNorte, in Teresina - PI (5 ° 5 '20'' S, 42 ° 48' 7'' W), located in North Central Piauiense region, where it was built a core conservation and multiplication of chicken ecotypes composed of naturalized areas of characterization, multiplication and evaluation, we also have support from laboratories of molecular biology and biotechnology, bromatology and animal nutrition and reproduction of birds. During validation System Alternative Creation Bird Grits (SACAC), developed by the same research group, found four isolated populations of chickens that had significant differences, which were named by the source Graúna Dourada (Itapecuru Mirim / MA), Brejeira (Brejo de Anapurus / MA), Teresina (Teresina / PI) and Nordestina (Regeneração / PI). Ecotypes were subjected to methods of morphological, morphometric, genetic and productive. For the ecotypes were defined management practices specific to nutrition, reproduction, health condition and housed in appropriate facilities and equipment. We also defined a plan of conservation and multiplication of genetic resources and technology diffusion. Every technology has been improved from the optimization of microsatellite markers previously used in industrial strains of chickens. It was then possible to detect genes responsible for quantitative and qualitative characteristics desirable than allowing the preservation and multiplication of ecotypes, their inclusion in breeding programs. Keywords: birds naturalized, genetic resources, genetic characterization, morphological, morphometric and productive.